上海雷浩地高辛DNA标记
地高辛DNA标记的详细资料：
地高辛DNA标记
比普通DIG随机引物法标记效率高,采用CSPO化学发光检测灵敏度更高.一个kit可用于12次标记反应和24次检测反应组成;5×高效Dig标记复合物;未标记对照DNA,PBR328;DNA稀释液;抗-Dig-碱性磷酸酶连接物;CSPD;10×封闭液;Dig定量试纸条;Dig对照试纸条;NBT/BCIP储存液,-20℃保存
Catalog#Product name/Packsize
11585614910
（1585614）DIG-High Prime DNA Labeling and Detection Starter Kit II
1 kit（12 labeling reactions and 24 detection reactions）
Application
DIG-High Prime labeled probes can be used in northern,Southern-,and dot-blot analysis,as well as colony and plaque hybridizations.This kit offers random-primed labeling of DNA templates with DIG-11-dUTP,alkali-labile,and chemiluminescent detection of the DIG-labeled hybrids.This kit was assembled with convenience in mind,offering ready-to-use CSPD supplied with a dripping device for easy application,ready-made blocking solution,and DIG Easy Hyb granules.The DIG-High Prime mixture includes stabilized Klenow enzyme,nucleotides,primers,and reaction buffer,all in one convenient reagent.
Benefits
The High Prime method of labeling offers many benefits:in addition to the optimized labeling efficiency,plasmid,cosmid,and phage DNA,linearized or supercoiled can be used as templates,yielding the same labeling efficiency.
Product DeSCRJPTion
Sensitivity and specificity:Standard assay uses 1μg of linearized pBR328.It is usual to expect 0.8μg labeled DNA after 1 h of labeling,and 2.3μg labeled DNA after 20 h reaction.In a dot blot hybridization,0.03 pg of homologous DNA is detectable with chemiluminescence（probe concentrations at 20 ng/ml）.A single copy human gene（t-PA）is detectable in a Southern blot,loading 1μg of digested placenta DNA.
Contents
DIG-High Prime,5x conc.,50μl
DIG-labeled Control DNA,20μl,（5μg/ml）pBR328（linearized with Bam HI）
DNA Dilution Buffer,3 x 1 ml
Anti-digoxigenin-AP Conjugate,50μl
CSPD,ready-to-use,50 ml
Blocking Solution,10x conc.,4 x 100 ml
DIG Easy Hyb Granules,4 x 100 ml
地高辛DNA标记
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